Glossary entry

Portuguese term or phrase:

com a visualização da expressão

English translation:

expression [of peptides] was observed

Added to glossary by liz askew
Nov 17, 2011 18:18
12 yrs ago
Portuguese term

com a visualização da expressão

Portuguese to English Other Biology (-tech,-chem,micro-)
A expressão da proteína foi verificada em gel SDS-PAGE 15% corado com Coomassie Brilliant Blue com a visualização da expressão de peptídeos de massa molecular de aproximadamente 19KDa, fusionado a resíduos de histidina (His-Tag).
Change log

Dec 1, 2011 19:16: liz askew Created KOG entry

Proposed translations

+1
1 hr
Selected

expression [of peptides] were observed

I think you need to change the sentence round a bit

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Note added at 1 hr (2011-11-17 19:35:54 GMT)
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www.patents.com/us-20090215113.html - Translate this page
... Tris/glycine SDS-PAGE and subsequent Coomassie Brilliant Blue staining. ..... G. Peptide Production By Bacterial Expression [0097]The peptides which .... Mercaptoethanol, 12.5% Glycerol, 0.005% Bromphenol Blue) to elute bound PT. ..... Ala Ser Ala Ile 50206PRTArtificial SequenceSynthetic histidine tag 20His His ...

of course that should be


expression.......................WAS observed


depends on how you parse the English
Peer comment(s):

agree Muriel Vasconcellos : Or 'could be obeserved' or 'was seen' (I would use the singular)
3 hrs
Thank you!
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3 KudoZ points awarded for this answer.
5 hrs

peptides were visualized

Detergent binding explains anomalous SDS-PAGE migration of ...
www.pnas.org/content/106/6/1760.fullby A Rath - 2009 - Cited by 77 - Related articles
A further proviso for MW estimation by SDS-PAGE is a consistent amount of ... **Peptide segments** that contain an abundance of hydrophobic ..... ...TM3/4 hairpins [uma proteína mutante] were expressed and purified as previously described (13). ...

um trecho do artigo acima:

**Proteins were visualized** with Coomassie Blue staining. Hairpin apparent MW values were estimated from the migration rates of Mark-12 markers (Invitrogen), ..

Em eletroforese com gel de poliacrilamida aka PAGE/SDS apenas os peptídeos/proteínas são visualizados como resultado da expressão do gene/genes estudado(s)... ou seja - não se visualiza a expressão, mas seu resultado.

HTH
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